Induction of diabetes in experimental animal rats were induced diabetes by the administration of simple intraperitoneal dose of alloxan monohydrate 150 mgkg nagappa et al. The anhydrous derivative ocnhco 2 co is also known as well as a dimeric derivative. Diabetes mellitus was induced by single intraperitoneal injection of 35. Protective effect of platycodin d on liver injury in alloxaninduced diabetic mice via regulation of tregth17 balance. Antioxidant effect of curcumin extracts in induced. This study evaluated the longterm effects of alloxan induced diabetes in rat liver. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. Differential effect of honey on selected variables in alloxan. Previously it has been reported that up780, a standardized composition of aloe chromone formulated with an aloe polysaccharide, has a significant impact in reducing hba1c, fasting blood glucose, fructosamine and plasma insulin level in humans and improved impaired glucose and insulin. Pdf alloxaninduced diabetes triggers the development of. This study aimed to determine the protective effect of curcumin on streptozotocin stzinduced oxidative stress in various tissues of albino wister rats. Alloxaninduced diabetes triggers the development of. Comparison of streptozotocin and alloxaninduced diabetes.
Oligosaccharides might contribute to the antidiabetic. Comparison of streptozotocin and alloxaninduced diabetes in. In the present study, we report the effects of induced diabetes and its treatment with or without oral hypoglycaemic drugs on the morphology and oxidative stress status of the prefrontal cortex. Effect of luff aegyptica leaf extract on body weigh in alloxan induced diabetic rats. The concentration of plasma insulin in alloxan induced diabetic rats decreased by 41% p in comparison with the control. The mechanism of alloxan and streptozotocin action in b. Antihyperglycaemic activity of cassia kleinii leaf extract. Research article alloxan induced diabetes mellitus in. Diabetes was induced in rats by administration of alloxan 150 mgkg, i. After the successful induction of experimental diabetes, the rats were divided into five groups each comprising a minimum of six rats. The concentration of plasma insulin in alloxaninduced diabetic rats decreased by 41% p induced diabetic rats treated with crude aloe vera gel 3.
In the presence of intracellular thiols, especially glutathione, alloxan generates reactive oxygen species ros in a cyclic redox reaction with its reduction product, dialuric acid. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. This study aimed to determine the protective effect of curcumin on streptozotocin stz induced oxidative stress in various tissues of albino wister rats. Antidiabetic activity of ethanolic extract of tubers of. Antidiabetic effect of morinda lucida stem bark extracts. Antihyperglycaemic activity of cassia kleinii leaf. Adult male rats 8 weeks, weighing 195 to 225 g was made diabetic by injecting stz 65 mg kg1 body weight intraperitoneally. However, there are draw backs to vanadate because it is poorly absorbed from the gastrointestinal tract and the doses of vanadate required for its antidiabetic effect are close to the. Metformin ameliorates insulitis in stz induced diabetic mice peerj. Adeniyi paulina oludoyin 1, 2, sanusi rasaki adegoke 1. Mice with acute diabetes induced by stz were administered metformin by gavage. Antidiabetic activity of vinca rosea extracts in alloxan. Rats were randomly assigned to the diabetic or control group.
Treatment of alloxaninduced diabetic rats with metformin or. I1 1department of medical laboratory science, school of basic medical sciences, university of benin, nigeria 2health services department, university of benin, nigeria 1. Diabetes was induced in wistar rats n 25 by intravenous administration of alloxan 42 mgkg and were analyzed at 1, 3, 6, 9 and 12 months after diabetes induction. May 24, 2014 there are a few nutritional approaches to address the increased needs of managing diabetic conditions. Moreover, spirulina supplemented to the diet or treatment with insulin increased the insulin concentration in plasma of diabetic rats by 53% p in alloxan induced diabetic rats for 14 days. Enhancement of bilirubin excretion in alloxan induced. The effect of alloxan diabetes on the activity of monoamine oxidase was studied in three regions of the rat brain at various time intervals after the onset of diabetes. Hyperglycemia, oxidative stress, liver damage and dysfunction. Mechanisms of inhibited lipolytic response to isoproterenol. Autoxidation of dialuric acid generates superoxide radicals. Glycemic effect of honey on alloxan induced diabetes and with concomitant administration of fructose was studied in male rats of the wistar strain.
The most frequently used intravenous dose of this drug to induce diabetes in rats is 65 mgkg b. Morfologia y enzimas renales alteradas en ratas con diabetes inducida por. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. Antihyperglycaemic activity of cassia kleinii leaf extract in glucose fed normal rats and alloxan induced diabetic rats v babu, t gangadevi, a subramoniam. A single dose of alloxan 120 mgkg body weight was used to induce diabetes in rats. Palliative effects of daonil supplemented with tryptophan on. Alloxaninduced diabetes model diabetes was induced by a single intraperitoneal injec. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. Phytochemical composition of the herbal extracts, fasting plasma glucose concentration. The hemimandibles were removed and submitted to radiographical and histopathological procedures. Single dose study after single dose of the mesc 200 or 400 mgkg, p. The rats are divided into 6 groups and each group con tains 6 rats n 6 as follows. Antihyperglycaemic activity of cassia kleinii leaf extract in glucose fed normal rats and alloxaninduced diabetic rats v babu, t gangadevi, a subramoniam.
Traditional plant medicines as treatment for diabetes. This study evaluated the longterm effects of alloxaninduced diabetes in rat liver. Diabetes was induced in groups 19 consisting of rats aged 3, 5, 7, 9, 11, 15, 17 and 19 weeks respectively by injecting 160 mgkg of alloxan monohydrate intraperitoneally while. The effects of alloxan on the histology of the pancreas. The animal care committee at the university of brasilia approved all procedures performed. In alloxan induced diabetic rats, when treated with the da extractmetformin, there was a significant p alloxan induced diabetes were found to be significantly p alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Alloxaninduced diabetes delays repair in a rat model of.
The current study sought to investigate the capacities of single and combinatorial herbal formulations of leaf extracts of acanthus montanus, asystasia gangetica, emilia coccinea, and hibiscus rosasinensis to reverse hyperglycemia and dyslipidemia in alloxaninduced diabetic male rats. We generated neonatallystreptozotocintreated female rats nstz and treated them with cnx01167, a gpr40 agonist to examine the role of gpr40 in modulation of glucose metabolism, insulin secretion and content. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. Thirty male albino rats were divided equally into three groups including control, diabetic and diabetic treated with kaysom extract. The mechanism of alloxan and streptozotocin action in b cells. Human islets are considerably more resistant to alloxan than those of the rat and mouse eizirik et al. Pdf antidiabetic effect of hydroalcoholic extract of carthamus. Original article modulatory influence of parkia biglobosa. Anti diabetic activity of ethanolic extract of aconitum ferox. Antidiabetic activity of methanol leaf extract of costus.
In animals, it can be induced by partial pancreatectomy or by the administration of diabetogenic drugs such as alloxan, streptozotocin, ditizona and antiinsulin serum. Thirty rats were used divided into 2 groups, normal control. Periodontal disease in diabetic patients presents higher severity and prevalence. Pdf antidiabetic and hypolipidemic effect of asparagus. In 1943, alloxan became of interest in diabetes research when dunn and mcletchie reported that it could induce diabetes in animals 4 as a result of the specific necrosis of the pancreatic beta cells 57.
Mechanisms of inhibited lipolytic response to isoproterenol by metformin in primary rat adipocytes this study is to investigate this study is to investigate the inhibitory effects of metformin on isoproterenol induced lipolysis in primary rat adipocytes and further elucidate the underlying mechanisms. Diabetic rats were administered kaysom extract orally twice daily for. Effect of combined dyslipidemia and hyperglycemia on diabetic. Taxifolin attenuates diabetic nephropathy in streptozotocininduced. Because of this, a potential pharmacodynamic interaction exists between these drugs and all antidiabetic agents.
It was observed that monoamine oxidase activity was decreased at early time intervals after diabetes, followed by a recovery in all three regions of the brain. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Introduction diabetes is a condition that arises when the. In 1838, wohler and liebig 1 synthesised a pyrimidine derivative, which they later called alloxan 2, 3. Diabetes was induced in groups b, c, and d using a single intraperitoneal injection of 150mgkg of alloxan after an overnight fast. The raw data has been supplied as a supplementary file. Hence it is vital to establish a dosage of drugs used in induction of experimental diabetes so as to develop stable diabetic characteristics with minimal drug toxicity. Metformin attenuated insulitis in the stzinduced mice model of diabetes. Alloxan, sometimes referred to as alloxan hydrate, refers to the organic compound with the formula ocnhco 2 coh 2. The mechanism of alloxan and streptozotocin action in b cells of the rat pancreas t.
Pdf silymarin increases antioxidant enzymes in alloxan. Gpr40 is a gprotein coupled receptor regulating free fatty acid induced and also glucose induced insulin secretion. Silymarin increases antioxidant enzymes in alloxan induced diabetes in rat pancreas. It has been found that in many research studies, the dose of alloxan used to induce diabetes was suboptimal. On the other hand, insulin treatment prevented diabetes induced alterations in astrocyte glutamate uptake and reverted the decreased gfap expression in rats at 4 and 8 weeks of diabetes duration 12. Thirty nondiabetic control rats nc and 30 untreated diabetic ud rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks.
Blood glucose level and lipid profile of alloxaninduced. Marked degenerations of the islets of langerhans was observed in pancreas of alloxan induced diabetic rats, whereas, histological examination of the pancreas of. Resiniferatoxin treatment reverses impaired capillary recruitment and insulin resistance in the obese zucker rat a recent unexpected report by a recent unexpected report by moesgaard et al. Diabetes was induced in albino rats by administration of single doses of alloxan monohydrate 120 mgkg, i. Samdialloxan induced diabetes in rats and the effects of black caraway carum carvi l. Effect of ginger zingiber officinale extracts on blood. Bumetanide, furosemide, and torsemide may cause hyperglycemia and glycosuria in patients with diabetes mellitus, probably due to diureticinduced hypokalemia.
Diabetes was induced in groups 19 consisting of rats aged 3, 5, 7, 9, 11, 15, 17 and 19 weeks respectively by injecting 160 mgkg of alloxan monohydrate intraperitoneally while group 10 rats. Pdf on oct 1, 2014, mohammad zoair and others published antidiabetic effect of artemisia annua kaysom in. Fresh liver weight and its relationship with body weight were obtained, and liver. Hyperglycaemic rats were treated with or without oral hypoglycaemic drugs metformin, 150 mgkgd. The rats were divided in to five groups of five animals each.
Composed from 10 rats and diabetic group composed from 20 rats i. Anti diabetic activity of ethanolic extract of aconitum ferox in alloxan induced diaseased rats j kondal reddy1, p sheetal2, rajesh menda2, nukala aparna3 1department of pharmacology, vision college of pharmaceutical sciences, boduppal, hyderabad, india. Induction of diabetes following an overnight fasting, diabetes was induced in the selected rats by a single intraperitoneal injection of freshly prepared stz sigmaaldrich co. Alloxan induced diabetic rats emili augustina mmaduaburochukwu1, osadolor h. Optimization of alloxan dose is essential to induce stable.
These are some of the earliest known organic compounds. Alloxaninduced diabetes, a common model for evaluating. Zingiberaceae leaf was investigated for its antidiabetic effect in wistar albino rats. Oligosaccharides might contribute to the antidiabetic effect.
They also exhibit a variety of biological activities. Dec 18, 2007 alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Twentyfour wistar rats weighing between 150 and 170g were used in this research work. Altered kidney morphology and enzymes in streptozotocin induced diabetic rats. Resiniferatoxin treatment reverses impaired capillary. Akpan et al enhancement of bilirubin excretion in alloxan induced diabetic rats treated with crude aloe vera gel 3. Antidiabetic effect of morinda lucida stem bark extracts on. If the inline pdf is not rendering correctly, you can download the pdf file here. Changes in monoamine oxidase activity in rat brain during.
Effect of ginger zingiber officinale extracts on blood glucose in normal and streptozotocininduced diabetic rats. Original article rat model of foodinduced nonobesetype. Fasting blood glucose levels there was no significant difference in the fasting blood glucose level of the animals before alloxan administration. Open access treatment of alloxaninduced diabetic rats. Effect of chronic treatment with bismaltolato oxovanadium. It has been successfully induced in a variety of animal species. Antioxidant effect of curcumin extracts in induced diabetic. During the whole experimental period, animals were fed with a balanced commercial chow and water. Fresh liver weight and its relationship with body weight were. Open access treatment of alloxaninduced diabetic rats with. Szkudelski department of animal physiology and biochemistry, university of agriculture, poznan, poland received november 2, 2000 accepted march 20, 2001 summary alloxan and streptozotocin are widely used to induce experimental diabetes in animals. During the first five days after diabetes induction, rats per group died because of alloxan toxicity. Group iii and group iv was diabetic rats received standard oral hypoglycaemic agent, glibenclamide 5mgkg and p. This is the reason why mild diabetes produced by alloxan recovers spontaneously in rats.
Dose dependent reduction in bgl, tc and tg was observed in alloxan induced diabetic rats treated with ethanol extract of smilax chinensis l. Rosmarinic acid ameliorates diabetic nephropathy in. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Novolog insulin aspart rdna origin dose, indications. Antidiabetic effect of morinda lucida stem bark extracts on alloxaninduced diabetic rats. Hyperglycaemia was induced in fasted wistar rats with alloxan 150 mgkg body weight. The mechanisms of alloxan and streptozotocininduced diabetes. Two days after alloxan injection, rats screened for diabetes having glycosuria and hypoglycemia with. Blood glucose lowering activity of aloe based composition. Alloxaninduced diabetes and bone repair 373 issn 0100879x.
Antihyperglycemic and antihyperlipidaemic effect of polygala. Fasting blood glucose levels there was no significant difference in the fasting blood glucose level. Diabetes was confirmed in rats 72 hours after stz administration by measur. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Altered kidney morphology and enzymes in streptozotocin. The best known druginduced diabetes model is the alloxan diabetes.
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